Figure 7.

LINE-1 ORF-1p promotes the metabolism or clearance of sorafenib in MHCC97-H cells.

Notes: (A and B) MHCC97-H cells were transfected with an empty vector or LINE-1 ORF-1p. (C and D) MHCC97-H cells were transfected with control siRNA or LINE-1 ORF-1p siRNA. Then, cells were treated using the IC₅₀ concentration of sorafenib for 12 hours (A and C) and harvested at the indicated time points. (B and D) MHCC97-H cells were injected into nude mice to form subcutaneous tumors. Sorafenib solution was injected into the tumors and tumor tissues were harvested at the indicated time points. Cell or tumor tissue samples were analyzed using LC-MS/MS to measure the remaining sorafenib. The concentration curve is shown as mean±SD. ^*P<0.05.

Abbreviation: LC-MS/MS, liquid chromatography–mass spectrometry/mass spectrometry.