Figure 7. Basal activity of wild-type and S707Y mutant PLCγ₂ is independent of protein phosphorylation at position 707.

COS-7 cells were transfected as indicated with 500 ng/well of either empty vector (Co., control) or increasing amounts (15, 50, 150, and 500 ng/well) of vector encoding either wild-type PLCγ₂ (WT), PLCγ₂S707Y (S707Y), PLCγ₂S707T (S707T), PLCγ₂S707C (S707C), PLCγ₂S707A (S707A), PLCγ₂S707D (S707D), or PLCγ₂S707E (S707E). Twenty four hours after transfection, the cells were incubated for 18 h with myo-[2-³H]inositol, and inositol phosphate formation was then determined.