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. 2018 Feb 6;9(76):34200–34212. doi: 10.18632/oncotarget.24409

Figure 3. Carnosol directly binds to RSK2.

Figure 3

(A) Modeling of carnosol binding with the N terminal domain (NTD) at the ATP binding pocket (i) of RSK2. Ligand Interaction Diagram (LID) of the binding (right panel). (B) Modeling of carnosol binding with the C terminal domain (CTD) at the ATP binding pocket (left panel) of RSK2. Ligand Interaction Diagram (LID) of the binding (right panel). The NTD RSK2 and CTD RSK2 structures are shown inas ribbon representation and carnosol is shown as stick representation. Carnosol directly binds to RSK2 using a (C) recombinant protein or (D) gastric cancer cell lysates. The recombinant protein or cell lysate was incubated with carnosol-conjugated Sepharose 4B beads or with Sepharose 4B beads alone. The pulled down proteins were analyzed by Western blotting. Similar results were obtained from 3 independent experiments.