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. 2018 Feb 6;9(76):34200–34212. doi: 10.18632/oncotarget.24409

Figure 4. Carnosol induces G2/M phase arrest.

Figure 4

(A-B) The effect of carnosol on cell cycle was examined in HGC27 or BGC803 gastric cancer cells. Cells were synchronized by serum starvation for 24 h and treated with serum and/or carnosol for 24 h in 10% serum and medium. Cells were stained with propidium iodide(PI) and cell cycle was analyzed by Fluorescence Activated Cell Sorting (FACS). (C) The effect of carnosol on cell cycle marker proteins was determined. Synchronized cells were treated with serum and/or carnosol for 24 h in 10% serum and analyzed by Western blotting. Band density was measured using the Image J (NIH) software program. For (A–C), similar results were observed from 3 independent experiments.