Skip to main content
. 2018 Feb 6;9(76):34200–34212. doi: 10.18632/oncotarget.24409

Figure 5. Effect of carnosol on gastric cancer cell apoptosis.

Figure 5

(A) Carnosol induces cell death. Cells were seeded in a 6-well plate and treated with carnosol at the indicated doses for 72 h. The number of suspended or attached cells was determined using a hematocytometer. (B–C) Carnosol induces apoptosis. Cells were seeded with carnosol in medium supplemented with10% FBS and then incubated for 72 h. Cells were stained with annexin V and propidium iodide(PI) and apoptosis was determined by Fluorescence Activated Cell Sorting (FACS). For (A–C), data are shown as mean values ± S.D. (n = 3) and the asterisk (*) indicates a significant (p < 0.05) difference compared to untreated control cells. (D) Carnosol strongly induces expression of apoptotic marker proteins. Cells were treated with carnosol for 72 h and the levels of caspase 9, cleaved caspase 7 and BcL-xL proteins were determined by Western blotting using β-actin as the loading control. Similar results were observed from 3 independent experiments. Band density was measured using the Image J (NIH) software program.