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. 2018 Sep 28;9(76):34240–34258. doi: 10.18632/oncotarget.26045

Figure 3. Sensitivities to HSP90 inhibitors in Ba/F3 cells expressing various amino acid substitutions of D835 on FLT3-ITD.

Figure 3

(A) Cells were treated with the increasing concentrations of quizartinib (1–256 nM) for 4 days, and the resulting cell viabilities were determined by WST-8 assay. The data are presented here as the mean ± SD from three independent experiments. (B) Cells were treated with or without 10 nM quizartinib for 6 h, and lysates of these cells were subjected to immunoblotting using the indicated antibodies. (C) Cells were treated with increasing concentrations of 17-AAG (upper; 4–1024 nM) or 17-DMAG (lower; 4–1024 nM) for 4 days, after which cell growth inhibition assays were performed as described in (A). (D) Cells were treated with or without 100 or 300 nM 17-AAG for 6 h, and lysates of these cells were subjected to immunoblotting using the indicated antibodies.