Table S2.
Sequences of PCR primers for target gene detection
| Gene | Sequence | Size (bp) | Tm (°C) |
|---|---|---|---|
| β-actin | F: 5′-CACGAAACTACCTTCAACTCC-3′ R: 5′-CATACTCCTGCTTGCTGATC-3′ |
265 | 60 |
| SALL4 | F: 5′-TCGATGGCCAACTTCCTTC-3′ R: 5′-GAGCGGACTCACACTGGAGA-3′ |
142 | 60 |
| TGF-β1 | F: 5′- CACACTGCAAGTGGACATC-3′ R: 5′- GCAGAAGTTGGCATGGTAG-3′ |
277 | 55 |
| E-cadherin | F: 5′-CGCATTGCCACATACACTCT-3′ R: 5′-TTGGCTGAGGATGGTGTAAG-3′ |
252 | 55 |
| N-cadherin | F: 5′-AGTCAACTGCAACCGTGTCT-3′ R: 5′-AGCGTTCCTGTTCCACTCAT-3′ |
337 | 55 |
| Vimentin | F: 5′-GAGCTGCAGGAGCTGAATG-3′ R: 5′-AGGTCAAGACGTGCCAGAG-3′ |
344 | 55 |
| Slug | F: 5′-CCTGGTTGCTTCAAGGACAC-3′ R: 5′-TCCATGCTCTTGCAGCTCTC-3′ |
395 | 55 |
| Twist | F: 5′-ACGAGCTGGACTCCAAGATG-3′ R: 5′-GGCACGACCTCTTGAGAATG-3′ |
484 | 55 |
| TGF-β1 (ChIP-PCR) |
F: 5′-CATGGGAGGTGCTCAGTAAA –3′ R: 5′-CAGGCGGAGAAGGCTTAAT-3′ |
235 | 55 |
Abbreviation: ChIP, Chromatin immunoprecipitation.