Figure 5.

CMAS expression is required for tumor initiation.

Notes: (A) qRT-PCR and (B) immunoblot indicating BPLER-shCMAS cells have decreased CMAS expression. 5×10⁵ BPLER-EV or BPLER-shCMAS cells were injected subcutaneously into the flanks of 4-week-old NOD/SCID/IL2Rγ^null mice (N=4/group) and (C) tumor size was recorded every 3 days. The average tumor volume and SD for each group of mice are shown. No palpable tumors were detected in mice injected with BPLER-shCMAS cells during this time period.

Abbreviations: BPLER-EV, BPLER cells infected with control lentiviruses; BPLER-shCMAS, BPLER cells stably knocked down for CMAS after infection with lentiviruses encoding a CMAS shRNA; CMAS, cytidine monophosphate N-acetylneuraminic acid synthase.