Skip to main content
. 2018 Oct 9;10:4349–4361. doi: 10.2147/CMAR.S174856

Figure 3.

Figure 3

LIMD1 inhibits cell migration and invasion in GC cells.

Notes: (A–C). Cell migration assays of BGC823 and SGC7901 cells transfected with LIMD1 overexpression vector (A and B) and LIMD1si (C) are shown (40×). (D–F) Cell invasion assays of BGC823 and SGC7901 cells transfected with LIMD1 overexpression vector (D and E) and LIMD1si (F) are shown (40×). (G–I) LIMD1 alters EMT markers. Western blot analysis with the indicated antibodies in LIMD1-overexpressing (G and H) and LIMD1-downexpressing (I) GC cells is depicted. Cell morphology of BGC823 cells with altered LIMD1 is depicted in (J) (10×). Immunofluorescence assay of EMT markers, including E-cadherin, N-cadherin, and Vimentin, is shown in (K) (40×). *P<0.05; **P<0.01; ****P<0.0001. LIMD1, LIMD1 overexpression vector; pc3.1, empty vector.

Abbreviations: EMT, epithelial to mesenchymal transition; GC, gastric cancer; LIMD1si, LIMD1-siRNA; NCsi, negative control siRNA.