Figure 6.

Mk3 deficiency leads to an increased expression of IFN-γ by activated NK and T cells after IAV infection, but does not affect the total number of lung-infiltrating cells. C57Bl/6 WT and Mk3^−/− mice were infected with 300 particles of IAV PR8. At 1, 3, 6, and 9 d p.i., the BAL fluid was analyzed for the content of the indicated cells by flow cytometry: total cell count (A); CD45.2⁺ (B); F4/80⁺ (C); CD3⁻/Dx5⁺ (D); CD3⁺/CD4⁺ (E); CD3⁺CD8⁺ (F); (G–I) intracellular IFN-γ⁺ NK-cell (G) and CD4⁺ (H) and CD8⁺ (I) T-cell subpopulations; and cytotoxic active NK cells (J). N = 3, with BAL cells pooled from 3 animals in each experiment. Statistical analysis, 2-way ANOVA.