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. 2010 Apr;24(4):1249–1260. doi: 10.1096/fj.09-147124

Table 2.

Methodology details for electrochemical-detector-based analysis

Laboratory Sample vol. Sample enrichment
3 50–100 μl 1) Bondelut CH column; 2) HPLC system 1; fraction containing 8-oxodG collected
5 50 μl Urine sample diluted 1:1 with 2 × MP1; HPLC system 1: fraction containing 8-oxodG collected (C1, MP1); HPLC system 2; fraction containing 8-oxodG collected (C2, MP2 and MP3)
7 500 μl (50 μl injected) SPE: bond elute C18(OH) (3 ml), bond elute strong cation exchange column (3 ml)
13 50 μl (10 μl injected) SPE: anion exchange column

Literature references for methodology are provided, where available. MP, mobile phase; C1, column 1; C2, column 2; C3, column 3.