Table 3.

Methodology details for immunoassay-based analysis

Laboratory	Sample vol.	Immunoassay conditions				Ref.
		Calibration (range)	Primary Ab and origin	Primary Ab incubation conditions	Assay source
3	95 μl	0.1–10 ng/ml	Clone N45.1 (JaICA)	Overnight, 4°C	In house, sample enrichment included	44⤻
8	50 μl	1.25–40 ng/ml	Clone N45.1	1.5 h, room temperature	In house	45⤻
10	25 μl	2.5–80 ng/ml	Clone 1F7	1.5 h, 37°C	In house
12 (ELISA 1)	50 μl	0.5–200 ng/ml	Clone N45.1	1 h, 37°C	New 8-OHdG Check ELISA (JaICA)
12 (ELISA 2)	50 μl	0.125–10 ng/ml	Clone N45.1	Overnight, 4°C	Highly sensitive 8-OHdG Check ELISA (JaICA)
19	50 μl	0.5–200 ng/ml	Clone N45.1	1 h, 37°C	Bioxytech 8-OHdG-EIA kit (Oxis Health Products Inc., Portland, OR, USA)	46⤻, 47⤻

Literature references for methodology are provided, where available. JaICA, Japan Institute for the Control of Aging (Shizuoka, Japan).