Figure 5.

Changes in the rate of protein synthesis, cellular protein content, and mRNA abundance during differentiation. A) Relative change in the rate of protein synthesis during differentiation was carried out by determining the initial rate of ³⁵S-methionine incorporation at each time point, as described in Materials and Methods. Time-point data represent means ± sd (n=3). B) Protein content of parasite cells during differentiation, as determined by Bradford assay, as described in Materials and Methods. Graph represents 1 of 2 independent experiments. C) Total cellular mRNA content during differentiation was determined described as in Materials and Methods. Graph represents 1 of 2 independent experiments.