Figure 4:

Aerobic scope (AS = MMR–RMR) for six tropical freshwater fishes measured during an acute exposure (16 h; circles) and following thermal acclimation (21 d; triangles) to three water temperatures. Data are shown as model estimates of the mean ± SEM. The effect of temperature on AS during the acute exposure was examined using a one-way repeated measures ANOVA that included temperature sequence group as a between-subject factor to control for the effect of treatment order on the responses, and its interaction with temperature treatment. For the post-acclimation data, temperature treatment effect on log₁₀ total AS was assessed using an ANCOVA with log₁₀ fish mass as a covariate. ANCOVA included ‘replicates’ nested within treatment to account for tank effect. For a given species, means with different letters differ significantly from each other within each exposure period (Holm–Sidak; P ≤ 0.05; Supplementary Tables S1 and S5). Upper case letters = acute exposure whereas lower case letters = post-acclimation.