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. 2018 Oct 15;13(10):e0205340. doi: 10.1371/journal.pone.0205340

Fig 1. TNF-α and IL-17A drive psoriasis transcript expression in ex vivo skin organ cultures.

Fig 1

To validate the use of cytokine-stimulated normal human skin biopsies as a model of skin inflammation, we treated biopsies with a combination of 10 ng/ml TNF-α and 20 ng/ml IL-17A for 12, 24, 48, and 72 h and monitored expression of DEFB4, PI3, LCN2, S100A7, and IL36G transcripts by qRT-PCR. Values are mean ± SD of results in triplicate from 3 donors. Statistical significance indicated by *p<0.05, **p<0.01, as compared with untreated cultures at each time point.