Fig 6. Rhodomyrtone inhibits IL-17A/TNF-α-induced inflammatory responses in keratinocytes.

Primary human keratinocytes were pretreated with rhodomyrtone (50, 100, 200, 400, and 600 ng/ml) for 1 h before stimulation with 20 ng/ml IL-17A+10 ng/ml TNF-α. HBD-2 (a), and CXCL-1 (b), were assayed in conditioned media at 24 h by ELISA and found to be reduced in a dose-dependent manner in the presence of rhodomyrtone. Values are mean ± SD of results from three independent experiments in triplicate. Statistical significance determined using one-way ANOVA and Dunnett’s multiple comparison test and noted ****p<0.0001, as compared with TNF-α treatment alone.