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. Author manuscript; available in PMC: 2018 Dec 15.

Published in final edited form as: Biochem Pharmacol. 2017 Sep 28;146:199–213. doi: 10.1016/j.bcp.2017.09.013

Figure 3. — Functional validation of novel miRNA response elements (MREs; shown in Figure 2) in the UGT2B7 and UGT2B15 3’-UTRs. For each miRNA evaluated luciferase reporter constructs were generated containing either the full length UGT2B7 (panels A-E) or UGT2B15 3’-UTR (panels F-T), a deletion (Δ) of the predicted MRE, or the triplicated MRE in the forward or reverse (negative control) orientation. Shown are relative luciferase activities (firefly/Renilla ratio normalized to the miRNA control) of HEK293 cells co-transfected with the luciferase reporter and either the miRNA mimic or the mimic negative control. Bars represent mean ± one S.D. from three independent experiments performed in quadruplicate. * p < 0.05, ** p < 0.01, *** p < 0.001.

Figure 3. — Functional validation of novel miRNA response elements (MREs; shown in Figure 2) in the UGT2B7 and UGT2B15 3’-UTRs. For each miRNA evaluated luciferase reporter constructs were generated containing either the full length UGT2B7 (panels A-E) or UGT2B15 3’-UTR (panels F-T), a deletion (Δ) of the predicted MRE, or the triplicated MRE in the forward or reverse (negative control) orientation. Shown are relative luciferase activities (firefly/Renilla ratio normalized to the miRNA control) of HEK293 cells co-transfected with the luciferase reporter and either the miRNA mimic or the mimic negative control. Bars represent mean ± one S.D. from three independent experiments performed in quadruplicate. * p < 0.05, ** p < 0.01, *** p < 0.001.