Table 5.

miRNAs identified to decrease the UGT2B15’-UTR-mediated luciferase reporter activity by at least 30% through library screening. Decrease in luciferase activity is represented relative to the average luciferase activities of non-transfected HEK293 cells and cells transfected with the miRNA mimic negative controls. In bold are the 12 novel candidate miRNAs identified in this study. Underlined are miR-376–3p and miR-331–5p which had been identified in a previous study by an in silico approach. Other listed miRNAs were not evaluated further since they showed a similar amount of inhibition of luciferase activity in a counter-screen of cells transfected with luciferase reporter lacking the UGT1A-3’UTR and so were considered false positives.

miRNA	Decrease in luciferase activity relative to the miRNA negative control (%)
miR-1262	62.3
miR-331–5p	62.1
miR-770–5p	54.4
miR-103b	53.9
miR-3942–5p	53.9
miR-3924	52.9
miR-30c-2–3p	50.1
miR-376b-3p	47.8
miR-376c-3p	46.4
miR-30c-1–3p	45.1
miR-455–5p	42.6
miR-605	42.6
miR-624–3p	41.6
miR-4712–5p	40.6
miR-3675–3p	40.6
miR-6500–5p	39.7
miR-548as-3p	38.7
miR-4292	37.9
miR-744–5p	35.5
miR-3678–5p	31.1