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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: Methods Mol Biol. 2018;1783:343–360. doi: 10.1007/978-1-4939-7834-2_17

Fig. 1.

Fig. 1

Mapping statistics (a) and (b) ChIP-Seq mapping statistics show mapped reads in green, multi-mapped reads in blue and unmapped reads in red. A high percentage of unmapped reads may indicate contamination with adapter dimers—see Fig. 2c. (c) and (d) RNA-Seq mapping statistics shows reads mapped to annotation in green, multi-mapped reads in blue, reads mapped outside annotation in orange and unmapped reads in red. High percentage of reads mapped outside annotation may indicate DNA contamination. (e) Ribosomal RNA contamination: High percentage of reads mapping to rDNA repeat indicate problems with mRNA isolation