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. Author manuscript; available in PMC: 2019 Oct 10.
Published in final edited form as: Cell Host Microbe. 2018 Oct 10;24(4):487–499.e5. doi: 10.1016/j.chom.2018.09.008

Figure 2.

Figure 2.

Trans complementation of the C7 mutant. (a) IFA after transfection. BHK-21 or BHKHA-C cells were transfected with C7 or WT RNA. On day 1 and 4 p.t., cells were fixed and stained for E protein expression (using 4G2 antibody). (b) IFA after infection. Supernatant from the RNA-transfected cells on day 4 p.t. was used to infect naive BHK-21 cells. At 24 h p.i., cells were fixed and stained for viral E protein expression. (c) Focus morphology. Viruses (C7t and WT) in the supernatants from transfected BHK-HA-C cells were subjected to focus-forming assay on BHK-21 and BHK-HA-C cells. On day 5 p.i., focus-forming units (FFU) were determined by immunostaining. (d) Virus titers in supernatants of transfected cells on day 4 post-transfection. The limit of detection is 10 FFU/ml. (e) Diagram of passaging C7t virus on BHK-HA-C cells. The C7t virus was continuously passaged on BHK-HA-C cells for 8 rounds, resulting in adaptive P8 C7t virus. (f) Focus morphology of P8 C7t virus on BHK-HA-C cells. Focus-forming units were assayed on day 5 post-infection. (g) Virus titer of P8 C7t virus. (h) Summary of the whole genome sequence of P8 C7t virus. The sequence of ZIKV strain FSS13025 (KU955593) was used as a reference.