Skip to main content
. Author manuscript; available in PMC: 2019 Oct 10.
Published in final edited form as: Cell Host Microbe. 2018 Oct 10;24(4):487–499.e5. doi: 10.1016/j.chom.2018.09.008

Figure 6.

Figure 6.

Robust T cell response in A129 mice after C7a/t immunization. Three-week old A129 mice were subcutaneously inoculated with C7a/t viruses (105 FFU) or DPBS (sham). Splenocytes were harvested on days 8 (a-e) and 49 (f-j) post-immunization, cultured ex vivo with ZIKV for 24 h or ZIKV E peptide for 5 h, and stained for IFN-γ, TNF-α, and T cell markers. Cytokines (IL-2, IFN-γ, and TNF-α) in splenocyte culture medium were measured by a Bioplex assay following ex vivo stimulation with ZIKV for 2 days. (a, f) Total numbers of CD4+ T cell subsets per spleen. Splenocytes were stimulated by ZIKV. (b, g) Total numbers of CD8+ T cell subsets. Splenocytes were stimulated by ZIKV (left panel) or E peptide (right panel). IL-2 (c, h), IFN-γ (d, i), and TNF-α (e, j) in cell culture were measured after splenocytes were stimulated by ZIKV. An unpaired nonparametric Mann-Whitney test was used for analyzing statistical significance.