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. 2018 Oct 15;13(10):e0205668. doi: 10.1371/journal.pone.0205668

Fig 3. The geNorm analysis for finding the optimal number of finger millet reference genes essential for precise and accurate normalization studies in all four sample sets.

Fig 3

The pairwise variation (Vn/Vn+1) was analyzed by the geNorm statistical tool to decide the minimum number of reference genes for precise and accurate RT-qPCR data normalization in each experimental set of finger millet samples. The cutoff value was 0.15; below this value indicates no additional reference gene required for RT-qPCR data normalization.