Table 1. Comprehensive details of the finger millet candidate reference genes, primer sequences, amplicon size, melting temperature (Mt), amplification efficiency (E); regression coefficient (R²), coefficient of variation (CV).

Ah—Arachis hypogaea; Ca—Cicer arietinum; Ec—Eleusine coracana; Pg- Pennisetum glaucum, Sb- Sorghum bicolor.

S. No	Gene	Name	Source/Acc No.	Primer sequence F / R (5'-3')	Amplicon Size (bp)	Mt	E	R2	Average Cq	CV (%)
1	ACT	Actin	Ec/HE800188	ATGAGGCCCAGTCCAAGAGA	168	84	1.03	0.997	24.44	8.17
				GGTTCAAAGGGGCTTCAGTG
2	CYP	Cyclophilin	Pg/KM105955	TACAAGGGGTCGAGCTTCCAC	104	89.7	1.08	0.922	27.02	5.0
				TTCTCGCCGTAGATGGACTCC
3	EIF4α	Eukaryotic Initiation factor 4A	Ca/XM_004513380	AGTCACTTCGGCCAGATTACAT	137	84.6	1.01	0.994	21.21	8.42
				AGCAGAGAAAACTCCCACTTGA
4	EF1α	Elongation factor 1-alpha	Ec/HQ202576	GCATGCTCTCCTTGCTTTCA	102	82.7	1.02	0.968	19.91	7.36
				TACTTGGGTGTGGTGGCATC
5	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Pg/GQ398107	TGCCTTGCTCCCCTTGCTAA	139	84.6	0.96	0.991	19.57	9.95
				CAGCCCTTCCACCTCTCCAG
6	G6PD	Glucose-6-phosphate 1-dehydrogenase	Ac/EG030635	ACCATTCCAGAGGCTTATGAGC	151	82.5	0.93	0.999	27.93	2.88
				AAGGGAGTGACTTGAACTCTCC
7	MACP	MalonylCoA-Acyl Carrier protein	Sb/XM_002465363	GCATTGAGAACATCGGGGCTT	139	84.6	1.00	0.996	26.51	6.95
				ATGAGTGGAAACTTCGTTCCA
8	MDH	Malate dehydrogenase	Sb/XM_002467034	TGCAGTGGTGGTGAATGGAA	103	83.7	1.01	0.994	26.61	8.04
				GCGTCTTCTCTTCCGACAGC
9	S24	40S ribosomal protein	Pg/KM105960	CCCCAGGAAGTGCTCTGCTA	158	86.1	0.97	0.987	25.79	7.36
				CATCAGCGTCACCCTGAGCA
10	PP2A	Serine/threonine-Protein Phosphatase	Ec/KT824869	GATCGCGTCCAAGAAGTTCC	109	83.1	0.98	0.996	24.32	7.97
				AAGTGTAGCCAGCACCACGA
11	PT	Phosphate transporter protein	Ec/KJ842585	GGCCTCTTCTCCCAGGAGTT	128	87.8	0.95	0.928	29.11	5.56
				TTGATGGCCGTGAAGATGTC
12	S21	Ribosomal protein	Ec-KC894816	ACTTCTACCCCGAGCACACG	152	84.4	0.97	0.994	15.27	13.96
				CGCTTATGACCTCCCCCTCT
13	TFIID	Transcription initiation factor	Ec-KT824872	ACCATGGATGGGTTCTCCAC	163	86.1	0.98	0.959	21.1	7.13
				GATCCTCCTTCCATGCTTGC
14	TIP41	Tonoplast intrinsic proteins -like protein	Ca/XM_004496854	GTTGTACTTCGGGAGAGTTGCT	115	83	0.95	0.956	30.33	2.47
				GGAGCTTCTGGCTTATGATGCT
15	β-TUB	Beta Tubulin	Pg/KM105955	CACCTCCATCCAGGAGATGTT	167	87.4	0.87	0.999	23.21	3.3
				GGTGAACTCCATCTCGTCCA
16	UBC	Ubiquitin Protein Isoform C	Pg/CD724586	TTCAAACCTCCGAAGGTGTCTT	100	81.7	0.88	0.998	23.26	6.07
				GGCTCCACTGCTCTTTAAGAATG