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. 2018 Oct 15;13(10):e0205586. doi: 10.1371/journal.pone.0205586

Fig 6. Botulinum neurotoxin and toxin-associated protein expression is highest in late phase growth.

Fig 6

C. botulinum (A) ATCC 3502, (B) ATCC 19397, and (C) ATCC 17841 express botulinum neurotoxin, non-toxin non-hemagglutinin (NTNH), and three hemagglutinin proteins (HA-17, HA-34, and HA-70), while (D) ATCC 35415 expresses botulinum neurotoxin, NTNH, and a different complex of associated proteins, including P-47, OrfX-2, and OrfX-3. The additional OrfX-1 protein was not detected in our experiments. Average protein abundance (spectral counts) of toxin complex proteins in cell and SN fractions are presented. Error bars denote standard deviation. Early time points (including OD600 = 0.4 and 0.8) are shown in white bars for cell fraction and gray bars for SN (note that all values were zero for early SN samples). The late time point (5 days) is represented in black bars for the cellular fraction and striped pattern for the SN fraction.