Table 2. Protein relative abundance ratios as signatures of cultivation conditions.
Ratio butyrate kinase: acetate kinase | ||
ATCC strain number | Exponential | Stationary |
3502 | 0.20 | 6.93 |
19397 | 0.20 | 2.17 |
17841 | 0.11 | 3.65 |
35415 | 0.13 | 6.30 |
3584 | 0.15 | 12.40 |
Ratio glycine reductase component B: 30S ribosomal subunit S13 | ||
ATCC strain number | Exponential | Stationary |
3502 | 0.17 | 3.62 |
19397 | 0.18 | 6.64 |
17841 | 0.11 | 5.33 |
35415 | 0.14 | 7.70 |
3584 | 0.18 | 3.19 |
Relative protein abundances in cell fractions were used to calculate two proof-of-principle ratios to distinguish samples from early or late phase growth. Each ratio was calculated using two proteins that were identified in all samples and were consistently enriched in either exponential or stationary phase growth.