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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Transpl Immunol. 2018 Jun 8;50:34–42. doi: 10.1016/j.trim.2018.06.003

Figure 2. Skin-graft-elicited Thy1.1+ CD8+ cells can be distinguished from those elicited by gHV infection based on KLRG-1 expression.

Figure 2

A) Representative flow cytometric staining of Thy1.1+ CD8+ antigen-specific T cells indicating the increased expression of KLRG-1 expression in gHV-primed Thy1.1+ CD8+ antigen-specific T cells as compared to similar skin graft-primed cells at day 9. B) Histogram representation of the increased expression of KLRG-1 in gHV-primed Thy1.1+ CD8+ antigen-specific T cells as compared to skin graft -primed cells at day 9, 16, and 30. C) Histogram representation of the increased expression of CD127 in gHV-primed Thy1.1+ CD8+ antigen-specific T cells as compared to skin graft -primed cells at days 9 and 30. D, Based on the gMFI, gHV-primed Thy1.1+ CD8+ antigen- specific T cells show increased expression of KLRG-1 as compared to skin graft -primed cells at day 9, day 16, and day 30. E), Based on gMFI, there is no difference in expression in CD127 at day 9 between gHV- and skin graft -primed antigen-specific Thy1.1+ CD8+ T cells, however skin graft -primed Thy1.1+ CD8+ antigen-specific T cells show increased expression of CD127 at memory. F) Histogram representation of increased KLRG-1 expression on Listeria monocytogenes (LM)-primed Thy1.1+ CD8+ antigen-specific T cells as compared to skin graft -primed cells at day 10. G) Graphical depiction of KLRG-1 expression by gMFI showing increased expression of KLRG-1 in LM-primed Thy1.1+ CD8+ antigen-specific T cells as compared to similar skin graft-primed cells at day. Representative of 3 independent experiments with 5-10 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant.