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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Transpl Immunol. 2018 Jun 8;50:34–42. doi: 10.1016/j.trim.2018.06.003

Figure 3. Cytokine profiles of skin graft- versus gHV-elicited antigen-specific Thy1.1+ CD8+ T cells are different at the peak of the response.

Figure 3

A) Splenocytes were harvested at day 9 and stimulated with PMA/Ionomycin. The frequency of IFN-γ and IL-2 producing cells was determined by flow cytometric staining. B) The results from figure 4A were quantitated. Skin graft-elicited antigen-specific Thy1.1+ CD8+ T cells show increased frequency of IL-2 producing cells and decreased frequency of IFNγ producing cells at day 9. Representative of 3 independent experiments with 5-10 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant.