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. 2018 Oct 15;9:4279. doi: 10.1038/s41467-018-06646-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Gene knockdown effects on surface CD1d and CD1d-lipid complex formation. a Effect on CD1d surface expression assessed by flow cytometry. The figure represents percentage change in mean fluorescence intensity (MFI) as compared to a non-targeting control. Average value from four experiments is shown. b Flow cytometry analysis of surface expression of αGalCer-CD1d complexes by J774-CD1d cells (live, singlets) after GalGalCer loading. Normalized MFI compared to non-targeting control, the average of four experiments is shown. Boxes represent the interquartile range; maximum and minimum values are displayed with vertical lines. p-Values are indicated (paired t-test). See also Supplementary Fig. 3, 4, 5 and 6