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. 2018 Oct 15;9:4279. doi: 10.1038/s41467-018-06646-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Effects on lipid antigen uptake to lysosomes. a Comparative plots of normalized values for CD1d expression, CD1d-lipid complex formation by flow cytometry, and IL-2 release as described above for indicated genes. b Labeled antigen uptake. APCs with the indicated genes knocked down were incubated with αGalCer-Bodipy for the indicated times and LysoTracker added for a 2 h chase. Figure represents the co-localization coefficient. Representative data from one of two experiments in which at least 100 cells per time-point were analyzed. Nonsignificant p-values are not indicated. c Representative images for αGalCer-Bodipy and LysoTracker at 2 h time-point. Scale bar 10 μm. Graphs represent mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001 (one-way ANOVA). See also Supplementary Fig. 7, 8, and 9