Fig. 2.

Aortic expression of BCL11B. (a) Bar chart showing reduction in BCL11B mRNA expression in cultured human aortic vascular smooth muscle cells is insignificant compared to PBMC and HEK293T cells. (b) Representative western blot of aortic BCL11B shows negative staining across nuclear and cytoplasmic homogenates of (I) low PWV samples, (II) high PWV samples, and (III) samples with the highest BCL11B mRNA levels. Positive control is protein lysate of HEK293T-expressing full-length BCL11B and negative control is protein lysate of HEK293T expressing an empty vector control. (c) Representative immunohistochemical staining of (n = 45) aortic samples shows lack of BCL11B expression in the aorta. Tonsil was used as a positive control for the antibody (scale bar = 50 μM)