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. 2018 Sep 26;10(9):97–109. doi: 10.4330/wjc.v10.i9.97

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©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Polymerase chain reaction genotyping and quantitative reverse transcriptase-polymerase chain reaction of NBCe1 mRNA. A: PCR analysis of DNA from tail biopsies, using primers flanking the proximal LoxP site, showing bands for the alleles in Slc4a4^+/+, Slc4a4^flx/+ and Slc4a4^flx/flx mice. The larger size of the band for the floxed allele is due to the presence of the LoxP site; B: Quantitative RT-PCR analysis of cDNA prepared from whole heart RNA (n = 6 for each genotype) showing a reduction of Slc4a4 mRNA in hearts of KO (Slc4a4^flx/flx^(Cre)) relative to WT (Slc4a4^flx/flx without Cre) mice. PCR: Polymerase chain reaction.