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. 2018 Oct 15;9(11):1051. doi: 10.1038/s41419-018-1106-8

Fig. 2. Plasminogen is needed for the histological changes in the skin after irradiation.

Fig. 2

a Representative images of hematoxylin and eosin (H&E)-stained skin sections from plg+/+ and plg−/− mice before and on day 9 after irradiation. Arrowheads and arrows point to the epidermis in plg+/+ and plg−/− mice, respectively. b The thickness of the epidermis in plg+/+ and plg−/− mice on different days after irradiation measured in H&E-stained skin sections. c Representative images of skin sections stained for CD31 (red) from plg+/+ and plg−/− mice on day 12 after irradiation. Arrowheads and arrows point to the blood vessels in plg+/+ and plg−/− mice, respectively. d Quantification of blood vessels in plg+/+ and plg−/− mice at different time points after irradiation. e Representative images of Ki67-stained (red) skin sections from plg+/+ and plg−/− mice on day 12 after irradiation. Arrowheads and arrows point to the proliferating cells in plg+/+ and plg−/− mice, respectively. f Quantification of proliferating cells in plg+/+ and plg−/− mice at different time points after irradiation. g Representative images of TUNEL (green) staining of skin sections from plg+/+ and plg−/− mice on day 1 and day 12 after irradiation. Arrowheads and arrows point to the apoptotic cells in plg+/+ and plg−/− mice, respectively. h Quantification of apoptotic cells in plg+/+ and plg−/− mice at different time points after irradiation. i Representative images of 8-oxo-2′-deoxyguanosine staining of skin sections from plg+/+ and plg−/− mice on day 1 and day 12 after irradiation. Arrowheads and arrows point to cells with oxidized DNA in plg+/+ and plg−/− mice, respectively. j Quantification of 8-oxo-2′-deoxyguanosine in plg+/+ and plg−/− mice at different time points after irradiation. For all quantifications, n ≥ 3 per genotype. In all immunostained sections, DAPI (blue) was used for staining the nuclei. Scale bar = 100 µm. *P < 0.05; **P < 0.01.