Fig. 3. Regressing C/EBPβ-deficient tumors display tumor-specific elevations in apoptosis and p53 protein, whereas adjacent C/EBPβ-depleted skin is unaffected.

Tumor bearing IKOβ mice were dosed with either vehicle control or tamoxifen at 19 weeks and tumors were collected 2 weeks later. a Deletion of C/EBPβ was confirmed by IHC staining. b Photograph displaying apoptotic cells (left) and quantification of apoptosis in H&E-stained tumors (right) (vehicle n = 18 tumors, tamoxifen n = 10 tumors). c Photograph displaying p53 IHC staining (left) and quantification of IHC staining for p53 (right) (vehicle n = 15 tumors, tamoxifen n = 12 tumors). d Quantification of apoptosis in H&E-stained adjacent normal epidermis (vehicle n = 5 mice, tamoxifen n = 8 mice). e Quantification of IHC staining for p53 in adjacent normal epidermis (vehicle n = 5 mice, tamoxifen n = 8 mice). f Quantification of p53 mRNA from tumors (vehicle n = 3 tumors, tamoxifen n = 3 tumor). g Quantification of IHC staining for p53 phosphorylated on serine 18 (vehicle n = 9 tumors, tamoxifen n = 11 tumors). Data are expressed as mean ± SD. *indicates significantly different from controls p < 0.05 via the Student’s t-test