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. 2018 Oct 15;4:48. doi: 10.1038/s41420-018-0111-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Treatment with OA for 7 days dose-dependently promoted the neural differentiation of NSCs, but suppressed astrocyte differentiation, as determined by western blotting (a, b) and immunofluorescence staining (c, d). a, b OA significantly and dose-dependently decreased the expression of Nestin and increased the expression of MAP2, but expression of GFAP was not significantly affected. c, d OA significantly and dose-dependently decreased the proportion of Nestin-positive cells and increased the proportion of MAP2-positive cells. Scale bars: 100 μm. All data are presented as means ± SD from three independent experiments. *p < 0.05 and **p < 0.01, compared with the Ctr group; ^ap < 0.05 and ^aap < 0.01, compared with 10 μM OA group; ^bbp < 0.01, compared with 20 μM OA group