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. 2018 Oct 15;8:15226. doi: 10.1038/s41598-018-33149-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Administration of NaHS BMMSC osteogenesis in vitro. (a) In vitro administration of H₂S donor (NaHS) in BMMSCs culture for 3 weeks. (b–d) Effect of NaHS on the BMMSCs osteogenesis, as assessed by MTT proliferation assay (b) ALP activity (c) and bone mineralization assay by Alizarin Red staining (ARS) (d). (e) Effect of HDAC3 activator ITSA-1 on the BMMSCs osteogenesis in vitro. (f) Collagen secretion on day 21 was studied by Sirius red assay. (g) Biochemical assay confirmed calcium accumulation during BMMSCs culture on day 14 and 21. (h,i) Western blot analysis of osteogenic proteins, RunX2 and OCN. Experiments were repeated three times. Data are expressed as mean ± SEM. n = 6 mice per group. *p < 0.05 compared with the WT mice, ^#p < 0.05 compared with the CBS^+/− mice.