Fig. 2.

Moesin ITAM signals in receptor-independent phagocytosis. a Syk or Moesin siRNA-transfected or NS control DC2.4 cells were immunoblotted with antibodies against corresponding proteins, and GAPDH (upper). The phagocytosis efficiency is compared to NS control (lower). n = 50, N = 3. b Cell lysates from DC2.4 cells were subjected to IP with anti-Moesin. Cells were treated with 1 µM Na₃VO₄ for 1 h before harvesting, with or without MSU (200 µg ml⁻¹) was added. N = 3. ***p < 0.001 by one-way ANOVA with Scheffé post hoc. c Cell lysates from DC2.4 cells transfected Flag-Syk-EGFP were subjected to IP with anti-Flag M2 beads. Cells were treated with 200 µg ml⁻¹ MSU crystal for indicated time before harvesting. N = 3. d DC2.4 cells were treated with 200 µg ml⁻¹ MSU crystal for indicated time. Total cell lysates were subjected to immunoblotting with the indicated antibodies. N = 4. e Cell lysates from Cos-1 cells transfected with Myc-Syk with or without Flag-Moesin were subjected to IP with anti-Myc. Some cells were treated with 200 µg ml⁻¹ MSU crystal for 1 h before harvesting. N = 3. f Schematics of Moesin and the three truncated Moesin fragments containing FERM domain (F), linker region (L), and C-terminal ERM-association domain (C). g Cell lysates from Cos-1 cells transfected with Myc-Syk and Flag-Moesin full-length and fragments were subjected to IP with anti-Flag M2 beads. Cells were treated with 1 µM Na₃VO₄ for 1 h before harvesting. N = 5