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. 2018 Oct 15;8:15248. doi: 10.1038/s41598-018-33568-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription of (a) ompR, (b) ompC and (c) ompF in wild type and OmpR mutant obtained in qRT-PCT. Relative gene expressions are shown in wild type E.coli (white column), in the absence of ompR (black column), wild type OmpR recovered in null ompR allele (hatched column) and G63S OmpR recovered in null ompR allele (grey column). Vertical error bars show the standard deviations of biological triplicates. The expression level obtained in wild type was taken as a reference for comparison. ^*P-value < 0.05; ^**P-value < 0.01; ^***P-value < 0.001.