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. 2018 Oct 15;8:15248. doi: 10.1038/s41598-018-33568-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation of wild type, G63S and D55A OmpR by truncated EnvZ. Around 1 µg of EnvZ was autophosphorylated by ³²P-ATP in lane 1. Approximately 0.28 µg of wild type OmpR (lanes 2–3), G63S mutant (lanes 4–5) or D55A mutant (lanes 6–7) were mixed with phosphorylated EnvZ. The reaction was terminated by adding stop buffer at 1 minute (lanes 2, 4 and 6), or 5 minutes (lanes 3, 5 and 7). The molecular weight ladder is marked on the right column. Full image is shown in Supplementary Figure S6.