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. 2018 Oct 15;8:15243. doi: 10.1038/s41598-018-33663-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concatenated phylogenetic analysis of MenFD (ML analysis). The tree is performed using IQ-TREE⁴⁸ under the C30 model⁴⁹. The tree was rooted manually, to facilitate comparison with the Bayesian tree (Fig. 3). The scale bar shows the inferred number of amino acid substitutions per site. Color codes are the same as in Fig. 3. The tree shows a clear relationship between Cyanidiophytina and Chlamydiales with bootstrap support = 85. The C30 model shows that Archaeplastida are monophyletic even if the statistical support values are very low for MenD and MenF and have a chlamydial origin. Note that MenD from Bacteriovorax is distantly related to the archaeplastidal sequences and only MenF shows this affiliation.