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. 2018 Oct 15;8:15276. doi: 10.1038/s41598-018-33215-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Tv1 and Tsu1.1 on activity and motor coordination of male Drosophila. Activity was recorded for five consecutive days using automated video tracking and measured as the distance travelled per hour (mm/h/fly). Controls were maintained during the same conditions as the teretoxin-injected flies with the only difference that they were injected with PBS. (A) Activity assay of Tsu1.1. (B) Activity assay of Tv1. (C) Climbing index measured the short term effects of the toxins as well as possible disruptions in their motor coordination. The climbing assays of Tsu1.1 revealed only trivial effects on climbing ability. (D) Climbing assay of Tv1, all at 20 μM and 100 μM. All error bars represent 95% CI. The numbers indicated below each bar denote the standardized effect size (g). The climbing index was done with at least 15–20 male flies in each vial. Control data are depicted with grey dots, and teretoxin injected-fly data with orange dots.