Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 15;8:15228. doi: 10.1038/s41598-018-33385-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Co-immunoprecipitation of PAD4 with the NCF1 and NCF2 subunits of NADPH oxidase. (a) Anti-NCF1 immunoblot of total lysate from resting neutrophils (lane 1), supernatant after immunoprecipitation with anti-PAD4 (lane 2), immunoprecipitates with anti-PAD4 (lane 3), supernatant after immunoprecipitation with anti-PAD2 (lane 4), or immunoprecipitates with anti-PAD2 antibodies (lane 5). (b) Anti-PAD4 immunoblot of anti-NCF1 immunoprecipitates (lane 1), supernatant after immunoprecipitation with anti-NCF1 (lane 2), or total lysate from resting neutrophils (lane 3). (c) Anti-PAD4 immunoblot of the samples in lanes 1–3 in panel a. (d) Anti-PAD2 immunoblot the samples in lanes 1, 4, and 5 in panel a. (e) NCF2 western blot of lysate from resting neutrophils (lane 1), anti-PAD4 immunoprecipitates, or the supernatant after immunoprecipitation (lane 3). (f) Anti-PAD4 immunoblot of anti-NCF2 immunoprecipitates (lane 1), supernatant after immunoprecipitation with anti-NCF21 (lane 2), or total lysate from resting neutrophils (lane 3). The data represent the results of five independent experiments.