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. 2018 Oct 15;8:15228. doi: 10.1038/s41598-018-33385-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization of PAD4 with gp91 and NCF1 in resting or activated neutrophils. (a) Confocal images of resting neutrophils (upper panels) or neutrophils stimulated with 25 nM PMA (lower panels) stained with anti-PAD4 (green) or anti-gp91 (magenta). (b) Confocal images of resting neutrophils (upper panels) or neutrophils stimulated with 25 nM PMA (lower panels) stained with anti-PAD4 (green) or anti-NCF1 (magenta). The fourth panels show the co-location of pixels of the two colors. The data represent the results of three independent experiments.