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. 2018 Oct 15;8:15228. doi: 10.1038/s41598-018-33385-z

Figure 5.

Figure 5

Reduced ROS generation in PAD4 knock-down HL60 cells. (a) Anti-PAD4 immunoblot of total cell lysates from HL-60 cells treated with medium alone (lane 1), or the two shRNAs targeting PAD4 (lanes 2 and 3). (b) Anti-PAD2 immunoblot of the same lysates. c) Anti-NCF1 immunoblot of the same lysates. (d) Anti-NCF2 immunoblot of the same lysates. (e) Anti-NCF4 immunoblot of the same lysates. (f) Oxygen consumption by parental HL-60 cells or HL-60 cells pretreated for 7 days with 2 different shRNAs targeting PAD4, as indicated, stimulated with 100 nM PMA. (g) Oxygen consumption by HL-60 pre-treated with medium alone, 1 µM ionomycin, 20 µM BB-Cl-amidine, or 25 µM GSK484, and then stimulated with 100 nM PMA. The data are representative of three independent experiments.