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. 2018 Oct 15;50(10):135. doi: 10.1038/s12276-018-0162-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunoblot analysis of TRIM25, PPARγ, and adipogenic markers during 3T3-L1 cell differentiation. Relative TRIM25 protein levels were measured using ImageJ (bottom graphs). b Real-time quantitative PCR analysis of the mRNA expression of TRIM25, PPARγ, and adipogenic markers during 3T3-L1 cell differentiation. c Immunoblot analysis of TRIM25 and PPARγ in white adipose tissue (WAT) of normal chow-fed (NC) vs. high-fat-fed (HFD) mice. d Immunoblot analysis of TRIM25 and PPARγ in WAT of control vs. ob/ob mice. Relative TRIM25 and PPARγ protein levels were measured using ImageJ (right graphs). e Pearson correlation coefficients between TRIM25 and PPARγ levels within human subcutaneous fat samples were calculated. All error bars shown are the s.e.m. (*p < 0.05, **p < 0.01, ***p < 0.001)