Table 3.
No. | Name | RT (min) | Formula | M ± X | Expected m/z | Experimental m/z | Error (ppm) | Area (%) |
---|---|---|---|---|---|---|---|---|
1 | Rutin | 7.727 | C27H30O16 | M + H | 611.1624 | 611.1607 | −2.87 | 3.659 |
2 | Kaempferol-3-glucoside | 7.931 | C21H20O11 | M + H | 449.1092 | 449.1078 | −3.04 | 1.542 |
3 | Quercetin | 7.010 | C15H10O7 | M + H | 303.0503 | 303.0499 | −1.24 | 1.674 |
4 | Valine | 0.790 | C5H9NO2 | M + H | 116.0713 | 116.0706 | −5.73 | 18.438 |
5 | Valine acid | 7.206 | C8H8O4 | M + H | 169.0481 | 169.0495 | 8.62 | 1.805 |
6 | TT amide | 9.064 | C18H17NO5 | M + H | 328.121 | 328.1179 | −9.13 | 4.977 |
7 | Tricin | 10.010 | C17H14O7 | M + H | 331.0821 | 331.0912 | −2.77 | 1.957 |
8 | 25(R)-Spirostan-3,6,12-trione/25(R)-Spirostan-4-ene-3,12-dione | 13.458 | C27H38O4 | M + H | 427.2881 | 427.2843 | −8.69 | 36.807 |
9 | Diosgenin | 11.836 | C27H42O3 | M + H | 415.3215 | 415.3207 | −3.15 | 0.260 |
10 | Tigogein | 12.553 | C27H44O3 | M + H | 417.3369 | 417.3363 | −1.34 | 0.431 |
11 | TT saponin A | 13.630 | C27H42O4 | M + H | 431.3196 | 431.3156 | −9.10 | 18.584 |
12 | Gitogenin | 13.950 | C27H44O4 | M + H | 433.3325 | 433.3312 | −3.00 | 1.946 |
13 | Palmitic acid monoglyceride | 14.477 | C19H38O4 | M + H/M + Na | 331.2847/353.2666 | 331.2843/353.2662 | −1.47/−1.17 | 0.070 |
14 | Phenylalanine | 5.731 | C9H11NO2 | M + COOH | 210.0767 | 210.0772 | 2.67 | 2.228 |
15 | N-trans caffeoyltyramine | 8.511 | C17H17NO4 | M − H | 298.1076 | 298.1085 | 3.11 | 1.325 |
16 | TT amide | 9.098 | C18H17NO5 | M − H | 326.1026 | 326.1179 | 2.50 | 1.613 |
17 | TT saponin | 9.253 | C30H26O13 | M − H | 593.1295 | 593.1301 | 0.88 | 0.831 |
18 | Kaempferol | 9.375 | C15H10O6 | M − H | 285.0405 | 285.0405 | −0.15 | 0.398 |
19 | Physcione | 10.044 | C16H12O5 | M + COOH | 329.0667 | 329.0612 | −0.21 | 0.374 |
20 | 7-Hydroxy-4′-methoxyisoflavone | 10.582 | C16H12O4 | M − H | 267.0655 | 267.0663 | 2.85 | 0.201 |
21 | Emodin | 12.000 | C15H10O5 | M − H | 269.0454 | 269.0455 | 0.40 | 0.071 |
22 | Kaempferol | 14.006 | C20H34O3 | M + COOH | 367.2488 | 367.2490 | 0.60 | 0.809 |
Abbreviations: m/z = mass-to-charge ratio; M = molecule; RT = retention time; TT = Tribulus terrestris; X = uncertain material.