Figure 3.

Strain-specific mucosal pro-inflammatory responses were observed during intestinal injury of mice colonized with commensal E. coli isolates. Cecal explant tissues from control ASF mice or ASF mice colonized with either commensal E. coli ST150, ST468, ST375, or ST129 were cultured overnight. Supernatants from these cultures were assessed by a magnetic multiplex assay (n = 5–16 animals per group) for levels of (A) GM-CSF, (B) IL-6, (C) IFN-γ, (D) IL-1β, (E) IL-17A, and (F) IL-33. All data were analyzed by a non-parametric Kruskal–Wallis test followed by unpaired Mann–Whitney post-hoc test. Values represent the mean ± SEM. Treatments with different letters are significantly different from one another at P < 0.05. *P < 0.05, **P < 0.01.