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. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307

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Copyright © 2018 Ao, Yao, Li, Yang, Dong, Zheng, Chen, Wu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of different components in the two-plasmid system on inducible cell killing. (A) Diagram of the events during genome editing. (B) Effects of Cas12a, targeting crRNA, donor DNA and λ-Red in the two-plasmid system. The data represent the means ± standard deviations from three measurements for each experiment.