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. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307

FIGURE 4.

FIGURE 4

Results of recombination at a single locus. The five loci were edited in five independent experiments. In each experiment, a single locus among the five loci was edited. The donor plasmids pTc-A2, pTc-G, pTc-L, pTc-M, and pTc-P provided donor DNA and crRNAs for the araD, galK, lacZ, mutS, and pyrF site, respectively. The helper plasmid p46Cpf1-OP2 was used. The data represent the averages of three independent experiments.