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. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307

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Copyright © 2018 Ao, Yao, Li, Yang, Dong, Zheng, Chen, Wu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombination efficiency of different CRISPR systems. The helper plasmid p46Cpf1-OP2 was combined with the donor plasmids pTc-P, pTc-PL, and pTc-GLP to perform simultaneous modifications at a single locus (pyrF), two loci (lacZ and pyrF) and three loci (galK, lacZ, and pyrF), respectively. For recombination based on CRISPR-Cas9, p46Cas9, pTs-P, pTs-PL, and pTs-GLP were used. The data represent the averages of three independent experiments.