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. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307

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Copyright © 2018 Ao, Yao, Li, Yang, Dong, Zheng, Chen, Wu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALA production by different strains. (A) A schematic of the genetically modified MG1655 strains. (B) Results of ALA production. 103 stands for the strain MG1655AX01 which carries a chromosomally integrated hem1 gene controlled by the 103 promoter; T7 stands for the strain MG1655AX02 which carries a chromosomally integrated hem1 gene controlled by the T7 promoter and a chromosomally integrated T7 RNA polymerase gene controlled by the native lac promoter; pLTT05 stands for the strain MG1655AX03 which harbors the plasmid pLTT05. pT7 + IPTG and pLTT05 + IPTG denote that IPTG was added during the fermentation of the corresponding strains.